What is a CMV promoter?
What is a CMV promoter?
The CMV promoter is a commonly used promoter for the production of high level recombinant protein in mammalian cells17. However, the expression level of the transgene driven by CMV promoter decreases with extended culture times because of transcriptional silencing, which is associated with DNA methylation18, 19.
How big is the CMV promoter?
Plasmid: CMV promoter
| Analyze: | Sequence |
|---|---|
| Size: | 508 |
| Notes: | Human cytomegalovirus (CMV) immediate early enhancer and promoter. |
| Stable: | Unspecified |
| Constitutive: | Unspecified |
How many genes does HCMV have?
The low-passage strain Merlin has become the reference strain for wild-type HCMV, and its sequence entry (NCBI GenBank accession NC_006273, Table 1) currently contains a set of 170 genes.
What is SV40 promoter?
The early promoter of the simian virus 40 (SV40) has been used as a model eukaryotic promoter for the study of DNA sequence elements and cellular factors that are involved in transcriptional control and initiation.
When is CMV promoter used?
The human cytomegalovirus (CMV) immediate-early enhancer and promoter is commonly used for transient expression of transgenes in ES cells. However, its use in the formation of stable cell lines is less common.
What is CMV gene?
The human cytomegalovirus (HCMV), whose genome is 235 ± 1.9 kbp long, is a common herpesvirus. However, the functions of many of its genes are still unknown. HCMV is closely associated with various human diseases and infects 60–90% of the global population.
What kind of genome does HCMV have?
Human cytomegalovirus (HCMV; species Human herpesvirus 5) has the largest genome of any known human virus, at 236 kbp in size (Dolan et al., 2004). The genome is a linear, double-stranded DNA molecule consisting of two unique regions, each flanked by inverted repeats.
How many CMV strains are there?
Forty-six HCMV strains, consisting of 2 laboratory strains, Towne and AD169, and 44 wild-type strains, isolated from 28 children and the mother of one congenitally infected child, were analyzed.
What is SV40 plasmid?
PSF-SV40 – SV40 PROMOTER PLASMID contains the Simian Virus 40 promoter upstream of the multiple cloning site (MCS) for expression in mammalian cells. Transcription termination is mediated by the SV40 poly-adenylation signals downstream of the MCS.
