What is an OT-1 mouse?

What is an OT-1 mouse?

Also Known As:OT-1 These mice contain transgenic inserts for mouse Tcra-V2 and Tcrb-V5 genes. These mice are often used to study CD8+ T cell response to antigen, positive selection, and in any research requiring CD8+ T cells of defined specificity. Like most TCR transgenics, these mice are somewhat immunodeficient.

What are ot i T cells?

These SIINFEKL-specific CD8+ T cells, commonly referred to as ‘OT-I T cells’, have a T cell antigen receptor that consists of α-chain variable region 2 (Vα2) and β-chain variable region 5 (Vβ5), which are inherited via a single transgene1.

What CD45 1?

Description. CD45. 1 is an alloantigen of CD45, expressed by Ly5. 1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets.

What is CD45 1 a marker for?

Allelic variants of the pan-haematopoietic cell marker CD45, identified as CD45. 1 and CD45. 2, have been established as a marker system to track haematopoietic cells following congenic mouse bone marrow transplants.

What is CD45 cells?

CD45 (lymphocyte common antigen) is a receptor-linked protein tyrosine phosphatase that is expressed on all leucocytes, and which plays a crucial role in the function of these cells. These CD45-associated molecules may play an important role in regulating CD45 tyrosine phosphatase activity and function.

Do antibodies have epitopes?

An epitope, also known as antigenic determinant, is the part of an antigen that is recognized by the immune system, specifically by antibodies, B cells, or T cells. The epitope is the specific piece of the antigen to which an antibody binds.

What is H-2Kb?

Abstract. The peptide-binding site of the murine MHC class I molecule H-2Kb contains a deep C pocket, that is critical for peptide binding, as it accepts the anchor phenylalanine or tyrosine residue located in the middle (position 5, P5F/Y) of H-2Kb binding peptides. H-2Kb predominantly binds octameric peptides.

What are CD45 1 mice?

CD45. 1 splenocytes come from C57BL/6 congenic mice that express the CD45. 1 allele. CD45 itself is a protein tyrosine phosphatase receptor expressed on all hematopoietic cells except mature erythrocytes and platelets and is encoded by the Ptprc gene.